Neutral dissociator, pH 7.0 |Cat# 20880000-1 |Size 50mL KT | $ 33.17
Antibody purification-Protein A kit |Cat# 21310000-1 |Size KT | $ 149
Reagents used for binding and purification of IgG using a Protein-A Column, followed by elution of purified IgG.
Kit contents:
- Protein-A Separopore® Column, 1ml
- Binding Buffer
- Elution Buffer
Antibody/ antigen puri-affinity buffer kit |Cat# 21310001-1 |Size KT | $ 55.67
Buffer reagents used for protein coupling and purification.
kit contains:
- Binding Buffer, 1L
- Elution Buffer, 500ml
Antigen-antibody dissociation kit |Cat# 21310002-1 |Size 50mL KT | $ 55.67
As part of the Antigen-Antibody Dissociating Kit, two reagents are provided; to dissociate your antigen-antibody complex from a column and neutralize dissociated antibody.
Contents:
femtoLUX™ Detection kit, No antibodies |Cat# 21310003-1 |Size KT | $ 185.47
- femtoLUX™ is a chemiluminescent detection system for Western Blots.
- It is based on an innovative formulation of isoluminol, supersensitive detection and washing buffers, and AB-horseradish peroxidase (HRP) conjugates.
- The femtoLUX™ immunodetection system is able to detect extremely low concentrations of antigens.
- Costs less than any other chemiluminescent system and provides longer and more intense light emission.
- Requires minimal antibody due to its extreme sensitivity.
- Supplied complete with a novel blocking agent, BLOT-QuickBlocker, which allows a rapid blocking step and produces a clear background.
- Includes detection reagents, detection & washing buffers, BLOT-QuickBlocker to perform detection on 1500cm2 membrane.
- A choice of conjugates and other reagents allow users the option to customize the system to precisely match their Western blotting requirements.
femtoLUX™ System, Goat IgG AB (rabbit) |Cat# 21310004-1 |Size KT | $ 395.67
femtoLUX™ System , Goat anti-Goat IgG H&L-HRP
femtoLUX™ is a chemiluminescent detection system for Western Blots. It is based on an innovative formulation of isoluminol, supersensitive detection and washing buffers, and AB-horseradish peroxidase (HRP) conjugates. The femtoLUX™ immunodetection system is able to detect extremely low concentrations of antigens. This economical system costs less than any other chemiluminescent system and provides longer and more intense light emission. It also requires minimal antibody due to its extreme sensitivity.
Specifications:
femtoLUX™ System- Human IgG AB (Goat) |Cat# 21310005-1 |Size KT | $ 395.67
femtoLUX™ System , Goat anti-Human IgG H&L-HRP
femtoLUX™ is a chemiluminescent detection system for Western Blots. It is based on an innovative formulation of isoluminol, supersensitive detection and washing buffers, and AB-horseradish peroxidase (HRP) conjugates. The femtoLUX™ immunodetection system is able to detect extremely low concentrations of antigens. This economical system costs less than any other chemiluminescent system and provides longer and more intense light emission. It also requires minimal antibody due to its extreme sensitivity.
Specifications:
femtoLUX™ System- Mouse IgG AB (Goat) |Cat# 21310006-1 |Size KT | $ 395.75
femtoLUX™ System, Goat anti-Mouse IgG H&L-HRP
femtoLUX™ is a chemiluminescent detection system for Western Blots. It is based on an innovative formulation of isoluminol, supersensitive detection and washing buffers, and AB-horseradish peroxidase (HRP) conjugates. The femtoLUX™ immunodetection system is able to detect extremely low concentrations of antigens. This economical system costs less than any other chemiluminescent system and provides longer and more intense light emission. It also requires minimal antibody due to its extreme sensitivity.
Specifications:
femtoLUX™ System- Rabbit IgG AB (Goat) |Cat# 21310007-1 |Size KT | $ 395.75
femtoLUX™ System , Goat anti-Rabbit IgG H&L-HRP
femtoLUX™ is a chemiluminescent detection system for Western Blots. It is based on an innovative formulation of isoluminol, supersensitive detection and washing buffers, and AB-horseradish peroxidase (HRP) conjugates. The femtoLUX™ immunodetection system is able to detect extremely low concentrations of antigens. This economical system costs less than any other chemiluminescent system and provides longer and more intense light emission. It also requires minimal antibody due to its extreme sensitivity.
Specifications:
femtoLUX™ System- Rat IgG AB (Goat) |Cat# 21310008-1 |Size KT | $ 395.67
femtoLUX™ System , Goat anti-Rat IgG H&L-HRP
femtoLUX™ is a chemiluminescent detection system for Western Blots. It is based on an innovative formulation of isoluminol, supersensitive detection and washing buffers, and AB-horseradish peroxidase (HRP) conjugates. The femtoLUX™ immunodetection system is able to detect extremely low concentrations of antigens. This economical system costs less than any other chemiluminescent system and provides longer and more intense light emission. It also requires minimal antibody due to its extreme sensitivity.
Specifications:
Immunomax kit |Cat# 21310009-1 |Size 100KT | $ 93.67
ImmunoMax Kit
ImmunoMax Kit is perfect for maximum antigen antibody reactivity in solution, cryofixed sections and paraffin embedded sections. Modern immunohistochemical detection methods are based on sandwich techniques such as, horseradish peroxidase (HRP), conjugated anti-HRP antibody complex (PAP), alkaline phosphatase (AP), conjugated anti-AP antibody complex (APAAP) or avidin-biotin complex (ABC) techniques. These techniques use bridging antibodies to increase signals. However, these methods suffer from low sensitivity in detecting many important antigens particularly when used in formalin-fixed and paraffin-embedded tissues.
The retrieval of many antigens that are masked in formalin-fixed tissues still left a significant number of antigens that could be detected even after enforced antigen retrieval in formalin-fixed, paraffin, embedded tissues. To circumvent these limitations, we have developed a single amplification technique, which allows the signal to be enhanced significantly above the low background.
Up to 200-fold signal amplification was achieved by covalent deposition of biotin reporter molecules, we combined and modified the retrieval and amplification steps and a new maximized immunohistochemical staining protocol, which is termed the ImmunoMax Method. This method enhances the sensitivity of the reaction up to 1000-fold compared with conventional immunohistochemical reactions. Antigens previously thought to be nonreactive or insufficiently detectable in formalin-fixed, paraffin-embedded tissues can now be detected using the ImmunoMax kit.
Aldehyde cross-linking generally camouflages a number of antibody binding sites. If pretreatment steps (proteases, microwave, chaotropic substances) allow the retrieval of varying number in these antibody binding sites, a system that strongly amplifies standard immunohistochemical procedures should allow the detection of these partly demasked antigens.
In the ImmunoMax procedure, enhancement is dependent on the deposition of biotinylated tyramine on the tissue by the catalytic action of HRP. Consequently, the covalently bound biotin molecules themselves act as binding sites and may further be \"new\" secondary, covalently bound antigen at the site of the primary antigen around the peroxidase activity. A single, biotinylatedd tyramine enhancement step allows primary antibodies to be used at a dilution up to 500 times higher than the dilurion used in the standard ABC technique. Furthermore, the signal can be enhanced to a level comparable to or even better than the signal obtained on cryostat sections, when optimized AR steps are included.
Quick-immuno-detect kit |Cat# 21310010-1 |Size 500ASSAY KT | $ 188.4
Quick-Immuno-Detect Kit
For rapid detection of antigens in any purification protocol, apply 1μL of sample to the strips provided. Allow the strips to react with your primary (rabbit) antibody for 5 minutes. Apply the Quick-Immuno-Detect reagent provided and watch for the developing color change.
Specifications:
Spectrophotometers widely in clinical laboratories as well as industrial and environmental laboratories are used in educational institutions.Spectrophotometer cuvettes
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